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Recombinant Biotinylated Human SIRP alpha V4 Protein,His-Avi Tag

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分子別名（Synonyms）	MFR; BIT;CD172a; MYD-1
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）	Biotinylated Human SIRP alpha V4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu31-Arg370(E33G, L44S, T50S, T52I, R54H).[Accession \| P78324 variant 4]
分子量大?。∕olecular Weight）	The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result.
內(nèi)毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
純度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized Human CD47, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V4, His Tag with the EC50 of 0.18μg/ml determined by ELISA.
制劑（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重構(gòu)方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

儲(chǔ)存條件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事項(xiàng)

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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