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Recombinant Human B7-H3/CD276 Protein, His Tag

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翌圣生物科技(上海)股份有限公司更新時(shí)間：2024-12-24 09:03:45

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詳細(xì)介紹

B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for regulating immune responses. Other family members include B7-1, B7-2, B7-H2, PD-L1 (B7-H1), and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share about 20-40% aa sequence identity.

產(chǎn)品性質(zhì)

Synonyms	B7H3; B7-H3; CD276; PSEC0249; UNQ309; PRO352; B7 homolog 3; CD276
Uniprot No.	Q5ZPR3-2
Source	HEK293 Cells-derived Human B7-H3/CD276 Protein,Leu29-Pro245 with His tag at the C-terminal
Molecular Weight	The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	> 95% as determined by SDS-PAGE and HPLC
Activity	ELISA Data：Immobilized Human B7-H3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC₅₀ of 29.4ng/ml determined by ELISA.
Endotoxin	< 1.0 EU per 1μg of the protein by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.