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Histone-H3 Mouse mAb 鼠單克隆抗體

品牌：翌圣生物
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翌圣生物科技(上海)股份有限公司更新時間：2024-12-24 09:03:45

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詳細介紹

組蛋白是真核生物中負責染色體纖維核小體結構的基本核蛋白。核小體由大約146 bp的DNA包裹在由四個核心組蛋白(H2A、H2B、H3和H4)每對組成的組蛋白八聚體上。染色質纖維通過連接蛋白H1與核小體之間的DNA相互作用進一步致密，形成更高階的染色質結構。該基因無內含子，編碼一個依賴復制的組蛋白，是組蛋白H3家族的成員。該基因的轉錄本缺少polyA尾部;相反，它們包含回文終止元素。該基因與6p22-p21.3染色體組蛋白基因集群中的其他H3基因是分開的。

產品性質

別名	H3 histone, HIST1H3A, Histone cluster 1, H3a
SwissProt ID	P68431
基因ID	8290
免疫原	Purified recombinant protein fragments expressed in E.coli.
緩沖液	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
分子量	Observed MW (kDa):15
克隆類型	Monoclonal Antibody
亞型	IgG1
宿主	Mouse
物種反應	Human,Mouse,Rat,Yeast
純化方式	Affinity Purified
細胞定位	Chromosome,Nucleus
研究領域	-

應用

稀釋比 WB: 1/2000-5000 ICC: 1/100-500 IP: 1/200 IHC: 1/50-300

	Western blot analysis of extracts from C6,3T3 and MCF7 cell lysates using Histon H3 Mouse mAb (1:20000 diluted).Predicted band size:17KDa.Observed band size:17KDa.
	IF analysis of Hela with DAPI (Left) and (Right) diluted at 1:200
	1 、 Input: Hela Cell Lysate 2 、 IP product: IP dilute 1:20 Western blot analysis: primary antibody : 1:2000,. Secondary antibody: Goat anti-Mouse IgG(H+L) (S1) 1:10000
	Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,Histone H3 Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tem- peRature, 30min). Negative control was used by secondary antibody only.
	Western blot detection of Histone H3 in C6,3T3,Hela cell lysates using Histone H3 Mouse mAb(1:10000 diluted).Predicted band size:15KDa.Observed band size:15KDa.
	Immunoprecipitation analysis of Hela cell lysates using Histone H3 Mouse mAb