Recombinant Human Survivin (HLA-A*02:01) Complex Tetramer Protein,His-Avi Tag

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Recombinant Human Survivin (HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and LMLGEFLKL peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&LMLGEFLKL]

分子量大?。?/font>Molecular Weight）

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per ug by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE

> 95% as determined by HPLC

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.