3D單分子熒光成像系統(tǒng)-SAFe 360

      SAFe 360是法國abbelight公司推出的一款基于單分子定位的顯微成像（SMLM）的新型3D單分子成像系統(tǒng)，它獨有的DAISY技術(shù)整合了散光技術(shù)和超臨界角光技術(shù)，能夠極大的提高定位精度，xyz三軸定位精度高達15nm，可以提供高清晰三維亞細胞結(jié)構(gòu)圖像，支持同時四色成像，可以用于細胞納米三維成像，觀測高清晰亞細胞器結(jié)構(gòu)，實時研究不同的結(jié)構(gòu)功能蛋白的共定位信息，在單分子水平研究分子動力學(xué)反應(yīng)以及細胞間的相互作用等。

設(shè)備參數(shù)

+ 成像模式：PALM、STORM、PAINT、smFRET 、SPT

+ 光源模式：Epi、TIRF、HILO

+ 分辨率：15 nm的XYZ軸分辨率

+ 超大視野：200 × 200 μm2的視野

+  一次可同時采集1.2 μm深度圖像信息

+  圖像深度：10 μm

+  實時漂移矯正

+  四色同時成像

+  活細胞成像模式

配套試劑

測試數(shù)據(jù)

發(fā)表文章

[1] Radhakrishnan, A. V., et al. "Single-Protein Tracking to Study Protein Interactions During Integrin-Based Migration." The Integrin Interactome. Humana, New York, NY, (2021). 85-113.

[2] Jouchet, Pierre, et al. "Nanometric axial localization of single fluorescent molecules with modulated excitation." Nature Photonics (2021): 1-8.

[3] Pernier, Julien, et al. "Myosin 1b flattens and prunes branched actin filaments." Journal of cell science 133.18 (2020).

[4] Jimenez, Angélique, Karoline Friedl, and Christophe Leterrier. "About samples, giving examples: optimized single molecule localization microscopy." Methods 174 (2020): 100-114.

[5] Mau, Adrien, et al. "Fast scanned widefield scheme provides tunable and uniform illumination for optimized SMLM on large fields of view." bioRxiv (2020).

[6] Orre, Thomas, et al. "Molecular motion and tridimensional nanoscale localization of kindlin control integrin activation in focal adhesions." bioRxiv (2020).

[7] Cabriel, Clément, et al. "Combining 3D single molecule localization strategies for reproducible bioimaging." Nature communications 10.1 (2019): 1980.

[8] Woodhams, Stephen G., et al. "Cell type–specific super-resolution imaging reveals an increase in calcium-permeable AMPA receptors at spinal peptidergic terminals as an anatomical correlate of inflammatory pain." Pain 160.11 (2019): 2641-2650.

[9] Belkahla, Hanen, et al. "Carbon dots, a powerful non-toxic support for bioimaging by fluorescence nanoscopy and eradication of bacteria by photothermia." Nanoscale Advances (2019).

[10] Denis, Kevin, et al. "Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease." Nature microbiology 4.6 (2019): 972.

[11] Szabo, Quentin, et al. "TADs are 3D structural units of higher-order chromosome organization in Drosophila." Science advances 4.2 (2018): eaar8082. 

[12] Boudjemaa, Rym, et al. "Impact of bacterial membrane fatty acid composition on the failure of daptomycin to kill Staphylococcus aureus." Antimicrobial agents and chemotherapy 62.7 (2018): e00023-18.

[13] Culley, Sian, et al. "Quantitative mapping and minimization of super-resolution optical imaging artifacts." Nature methods 15.4 (2018): 263.

[14] Berger, Stephen L., et al. "Localized myosin II activity regulates assembly and plasticity of the axon initial segment." Neuron 97.3 (2018): 555-570.

[15] Cabriel, Clément, et al. "Aberration-accounting calibration for 3D single-molecule localization microscopy." Optics letters 43.2 (2018): 174-177. 

[16] Bouissou, Ana?s, et al. "Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring." ACS nano 11.4 (2017): 4028-4040. 

[17] Sellés, Julien, et al. "Nuclear pore complex plasticity during developmental process as revealed by super-resolution microscopy." Scientific reports 7.1 (2017): 14732.

[18] Bourg, Nicolas, et al. "Direct optical nanoscopy with axially localized detection." Nature Photonics 9.9 (2015): 587.

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