Recombinant Human HPV16 E7 (HLA-A*02:01) Protein,His-Avi Tag

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Recombinant Human HPV16 E7(HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-

Met119(B2M) and YMLDLQPET peptide.[Accession | P04439-1(HLA-A*02:01)&P61769(B2M)

&YMLDLQPET peptide]

分子量大?。?/font>Molecular Weight）

The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.